Toxicity on Pollen germination. Received: 15th February 2012 Abstract: Pollen obtained from the Hulls Rosa-Slovenes, commonly known as the Hibiscus flower were exposed to different concentrations of Lead to understand the effect of heavy metal on pollen tube germination. Heavy metals such as Lead, have shown to decrease the pollen germination and inhibit pollen tube growth. There was a correlation observed between the concentration of lead the pollen was exposed to ND the extent of growth Inhibition observed.

The effect of 20 pimp, pimp, pimp and 80 pimp lead on pollen tube germination were studied using light microscopy. A progressive decrease in the percentage of pollen germination indicated that higher levels of toxicity caused higher growth inhibitions. Introduction: Heavy metals are natural components of the Earth’s crust. They cannot be degraded or destroyed. To a small extent they enter our bodies via food, drinking water and air. As trace elements, some heavy metals are essential to maintain the metabolism of the human body. However, at higher concentrations they can lead to poisoning for human, animal, plant and microorganisms.

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Over the last decades, environmental contamination with heavy metals has increased drastically (Hussar Koran, Emmet Shall 2005) Heavy metals have recently received the attention of researchers all over the world, mainly due to their harmful effects on plant. The toxic effects of metals have also been intensively studied at the level of biochemical- physiological process such as photosynthesis, transpiration, enzyme activity or metal accumulation in tissue. Pollen germination and tube growth are used to detect biological activity of various environmental pollutants such as pesticides, heavy metals, acid rain etc.

In this experiment we report the effect of deferent concentrations of lead, and Its effect on pollen germination as seen In the pollen grains of the Hibiscus flower. Materials: Chemicals : -Penny powder obtained from Lobo chemicals. -Solutions of deferent concentrations of Lead (20,40,60 and 80 pimp). Dilutions prepared using distilled water. -O. IN HCI obtained from Mere, Chronicles, Iambi. Glass ware: Glass slides, Moisture chambers, Petri dishes, Pipettes, Droppers. All Obtained from Borzois) Filter paper, Scalpel/Blade, Microscope (Metzger Pat Ltd. ), Hibiscus flowers.

Method : Pollen grains were obtained from the Hibiscus flower by placing the anthers on a glass slide and lightly dusting the pollen grains using a scalpel or a blade. The effect of each concentration was studied in triplicates, so as to minimize error. A negative control using dill. HCI and a positive control using sucrose solution were prepared for each set. Thus a total of 24 glass slides were prepared. Alongside, the filter paper Nas placed in a moisture chamber and wet with a few drops of water so as to keep he conditions moist and favorable for pollen germination.

The lead solution was diluted to obtain individual solutions of concentrations pimp (ml BP + ml DID), pimp (ml BP + DID), pimp (ml BP + DID) and pimp (ml BP +D/W). 0. 1 ml of 20 pimp lead solution was mounted on to 3 slides with pollen grains. 3 such slides were prepared for each dilution of lead solution prepared. 3 controls were prepared for each set, using dill. HCI, distilled water and sucrose as negative and positive controls respectively. Each of these slides were placed in the moisture chamber and allowed o stand for 20 minutes.