You correctly answered: d. A negative result with a positive control is required to validate the test 5. Which of the following is an end product of starch digestion? You correctly answered: e. Maltose and glucose 6. Hydroplanes are enzymes that break down large molecules into smaller subunits through the addition of water 04/08/15 page 1 Experiment Results Predict Question: . You correctly answered: b. Predict Question: What effect do you think boiling and freezing will have on the activity of the amylase enzyme? Your answer : c. Boiling will decrease amylase activity and freezing will have no effect.

Stop & Think Questions: Tube 2 appears to have the same amount of starch digested as tube 3 because You correctly answered: a. Freezing had no effect on the enzyme. Experiment Data: Tube NO. 2 3 4 Reagent 1 Amylase 5 Denizen Water pH 2. 0 Buffer 6 7 8 Reagent 2 Starch Reagent 3 pH 7. 0 Buffer PH 7. 0 Buffer Treatment Boiled Frozen None Time 60 SKI Benedicts 37 Maltose pH 9. 0 Buffer page 2 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Tubes 3, 7, and 8 reveal that You correctly answered: d. Amylase activity was highest at pH 7. 2.

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This activity includes a number of negative controls. Which tube indicates that the amylase solution was not contaminated with maltose? You correctly answered: b. Tube 4 3. Which tubes indicate that the denizen water did not contain contaminating starch or maltose? You correctly answered: c. Tubes 4, 5, and 6 4. Explain where and why salivary amylase would be most active. You correctly answered: d. Salivary amylase would be most active in the mouth because pH 7 is where its peak activity is. Page 3 Review Sheet Results 1. List the substrate and the subunit product of amylase.